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Created on Tuesday, 08 June 2010 12:52

Peroxide Value test in Fats and Oils

With the analysis systems in CDR FoodLab range, in just 4 minutes, without titration, using micro quantity of sample you can carry out the Peroxide Value test of edible Oils and fats like Olive oil, Peanut oil, Sunflower oil, castor oil, palm oil and the other vegetable oils, butter, margarine, Fish oil and so on. Absolutely no preparation for oil sample and a really easy one for fat sample. The method in compliant to AOCS Official Method Cd 8-53.

Features

  • Rapids results: 4 minute ISO/AOCS compliant method.
  • Simple method: photometric method, low toxicity reagents pre-filled in disposable cuvettes.
  • test using micro-samples:suitable even to test fat substances that have a difficult extraction process.
  • Cost effective solution: rapid return on investment

Take a look! It's fast and simple

 

Compliant to AOCS Method

CDR method shows a very good correlation with AOCS Official Method Cd 8-53.

peroxidevaluevsreference

 

Analyzers

You can determine the value of the Peroxide Value (PV) in oils and fats with different systems of FoodLab Line:

  • FoodLabFat designed for testing fats and oils: free fatty acids (FFA)/Acidity, peroxide value (PV), soaps, Anisidine value (AnV) in oils and fats
  • OxiTester: to test Free Fatty Acids (FFA), Peroxide Value (PV) and Polyphenols/Oil Stability Index (OSI) in vegetable oil like olive oil, avocado oil, seeds oil etc.
  • FoodLab: designed for testing food like milk, eggs, tomatoes, vegetable puree, cheese, dairy products and fats.
  • PalmOilTester designed for testing palm oil: acidity (FFA), Iodine Value (IV), DOBI & carotene content, Peroxide Value (PV), Anisidine Value (AnV) in palm oil.
  • WineLab designed for testing Wine and Must, configurable to determine FFA, Peroxide Value, poliphenols in olive oil.
  • MiniFood: to test Free Fatty Acids and Peroxide Value in oils and fats.

Peroxide Value AnalyzersTo find out what is the analyzer that best suits your needs
See the comparison table»



Method

Test type: End Point
Colorimetric reaction at 505 nm
Testing time: 4 minutes - 70 Tests/Hour
It is possible to carry out several test sessions with a maximum of 14 samples.
Calibration can be performed by aligning the test to reference values.

Principle of the test

R-O-O-R peroxides oxidize Fe2+ ions. The Fe3+ ions resulting from oxidation are grouped and form a red complex. Its colorimetric intensity, measured at 505 nm, is directly proportional to the concentration of peroxides in the sample. Results are expressed as meqO2/Kg.

The amount of peroxides of fats indicate the degree of primary oxidation and therefore its likeliness of becoming rancid. A lower number of peroxides indicates a good quality of oil and a good preservation status.

Unsaturated free fatty acids react with oxygen and form peroxides, which determine a series of chain reactions that generate the production of smelling volatile substances. Those reactions are accelerated by high temperature and by light and oxygen exposure.

CDR innovative method simplifies and speeds up the standard procedure, because it enables the analysis to be carried out using micro-quantities of samples and to be applied to fats that are difficult to extract.

 

Composition of the Kit and Reagents

Code *300150: kit suitable for 100 tests, contains :
1) 10 bags with 10 pre-filled cuvette with reagent R1 (mix of alcohols and chromogenous compounds).
2) 2 bottles with R2 reagent (redox solution).

Code *300159: suitable for 20 tests, contains:
1) 2 bags with 10 pre-filled cuvette with reagent R1 (mix of alcohols and chromogenous compounds).
2) 1 bottle with R2 reagent (redox solution).

Diluition Kit *300129 - suitable for 100 tests, contains:
1) Diluent: Bottle with 100 mL of diluent (mixture of alcohols).
2) 100 test cuvettes with caps.

Code *300150/A: suitable for 100 tests, contains:
1) 10 bags with 10 pre-filled cuvette with reagent R1 (mix of alcohols and chromogenous compounds).
2) 2 bottles with R2 reagent (redox solution).

Code *300159/A: suitable for 20 tests, contains:
1) 2 bags with 10 pre-filled cuvette with reagent R1 (mix of alcohols and chromogenous compounds).
2) 1 bottle with R2 reagent (redox solution). (OxiTester)

Code *300164: suitable for 10 tests, contains:
1) 1 bags with 10 pre-filled cuvette with reagent R1 (mix of alcohols and chromogenous compounds).
2) 1 bottle with R2 reagent (redox solution). (MiniFood)

Codice *300160: suitable for 100 tests, contains:
1) 10 bags of code * 300164

Code *300190: suitable for 100 tests, contains :
1) 1 bottle with R1 (mix of alcohols and chromogenous compounds).
2) 2 bottle with reagent R2 (redox solution).
3) 100 test cuvettes with caps (PalmOilTester)

Sample Handling and Usage of Curves

Sample
Oil as is (olive oil, extracted from seeds, nuts, etc.).
Solid fats: melted using bain-marie method before starting the test (animal fats, palm oil, etc.).
Extractable fats: extract the fatty part following the instructions provided in the test method (butter, cream, margarine, semi-finished products, etc.).

Peroxide Value Test in Olive Oil and Vegetable Oils:
Specific curves suitable for the analyzers OxiTester, OxiTester k270 e WineLab

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perox.oil extr *300150/A
*300159/A
0,3 - 25 5 µL 0.01 +/- 5% CV<3%
Perox.oil high *300150/A
*300159/A
1 - 50 2.5 µL 0.01 +/- 5% CV<3%

Specific curves suitable for the analyzer MiniFood.

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perox.oil extr *300160
*300164
1 - 50 2.5 µL 0.01 +/- 5% CV<3%

Peroxide Value in Essential Oils

Specific curves suitable for the analyzer OxiTester.

CurvaCodeMeas. range
(mmol/L)
Sample VolumeResolution
(mmol/L)
AccuracyRepeatability
Px ESS. OILS *300150/A
*300159/A
0 – 13 5 µL 0.01 +/- 5% CV<3%
Px OLII ESS.>> *300150/A
*300159/A
0.5 - 25 2,5 µL 0.01 +/- 5% CV<3%

Peroxide Value Test in fat matrix

Specific curves suitable for the analyzers FoodLab e FoodLabFat.

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perox. 50 µL *300150
*300159
0.01 - 3.4 50 µL 0.01 +/- 5% CV<3%
Perox. 25 µL *300150
*300159
0.1 - 5.5 25 µL 0.01 +/- 5% CV<3%
Perox. 10 µL *300150
*300159
0.5 - 11 10 µL 0.01 +/- 5% CV<3%
Perox. 5 µL *300150
*300159
0.3 - 25 5 µL 0.01 +/- 5% CV<3%
Perox. 2.5 µL *300150
*300159
1 - 50 2.5 µL 0.01 +/- 5% CV<3%
Perox.dil-100 µL *300150
*300159
4 – 275 5 µL diluted 1 +/- 5% CV<3%
Perox.dil-50 µL *300150
*300159
7 – 550 5 µL diluted 1 +/- 5% CV<3%

The curves 50µL and 25µL are mainly recommended for refined oils with very low Peroxide Value, requiring great precision to values lower than 1. The curve 5µL can be used for refined oils and non-refined with Peroxide Value below 25.

Peroxide Value Test in butter; margarine; cream and semi-finished fats

Specific curves suitable for the analyzers FoodLab e FoodLabFat.

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perox. 25 µL *300150
*300159
0.1 - 5.5 25 µL 0.01 +/- 5% CV<3%

The curve 5µL is the same for the analysis of fats, but in this case it is necessary to make a small sample treatment.

Peroxide Value Test in dried fruits nuts

Specific curves suitable for the analyzers FoodLab e FoodLabFat.

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perox. 25 µL *300150
*300159
0.1 - 5.5 10 µL 0.01 +/- 5% CV<3%
Perox. 10 µL *300150
*300159
0.5 - 11 5 µL 0.01 +/- 5% CV<3%

The curves are the same for the analysis of fats, these are the most suitable rangefor the application of dried fruit nuts. The oil is extracted by pressure through the system CDR cold extraction.

Peroxide Value Test in Palm Oil

Specific curves suitable for the analyzer PalmOilTester.

CurveCodiceMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
PV low refined *300190 0.01 - 0.55 25 µL 0.01 +/- 5% CV<3%
PV low refined *300190 0.3 - 11 10 µL 0.01 +/- 5% CV<3%
PV high refined *300190 0.5 - 25.4 5 µL 0.01 +/- 5% CV<3%
PV low crude *300190 0.5 - 24.7 5 µL 0.01 +/- 5% CV<3%
PV high crude *300190 1 - 60.5 2.5 µL 0.01 +/- 5% CV<3%

Peroxide Value Test in Flour

Specific curves suitable for the analyzer FoodLabFat.

CurveCodeMeas. range
(mEqO2/Kg)
Sample VolumeResolution
(mEqO2/Kg)
AccuracyRepeatability
Perossidi farine *300150
*300159

0,05 – 4,60
(with 100% fat)

100 µL
of solution
0,01 +/- 5% CV<3%

To carry out the analysis of peroxide in the flour is sufficient a very easy sample preparation.

Find out the analyzer that best suits your needs

Peroxide Value AnalyzersTo find out what is the analyzer that best suits your needs
See the comparison table»

Reagent developed by CDR S.r.l - ISO 9001 certified company



 
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