Determination of Glycerol in wine and must
The presence of polyhydric alcohols determines, in wine, enveloping sensations, also called roundness, which make the softness of the wine. Among the polyalcohols which are formed during the fermentation there are mannitol, sorbitol, butylene glycol and glycerol. The latter is particularly interesting because the wine can be present with values ranging between 5-18 g/L and is also produced in large quantities by noble rot during the production of passito wines. This explains the softness and elegance of this type of wine.
Method
Sample treatment
Wine is analyzed as it is. If necessary (sparkling or fermenting wine), the sample shall be degassed using the specific ultrasonic bath (code 222062). In case of tests performed on must, the sample shall be first centrifuged (code 222061). Dilute the sample with the included dilution kit, if required by the method.
Principle of the test
The glycerol reacts, by an enzyme, with a phenolic derivative and induces the formation of a complex pink color whose intensity, measured at 505 nm, is directly proportional to the concentration of glycerol in the sample. The result is expressed in g/L.
Calibration curve
The calibration curve of the CDR WineLab® instrument, carried out comparing to the reference method, confirmed an excellent linearity of the system over the entire measurement range.
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Reagent test Kits
Code*300517: 10 tests
Code*300520: 100 tests
Measuring range
| Analyses | Measuring range | Resolution | Repeatability |
|---|---|---|---|
| Glycerol [2-15] g/L | 2 - 15 g/L | 0.1 g/L | 0.2 g/L |
| Glycerol [50-800] mg/L | 50 - 800 mg/L | 1 mg/L | 11 mg/L |
Analyzers for process and quality control in winemaking
CDR WineLab®
Complete analysis panel, supplied already configured
Up to 16 determinations simultaneously
Possibility of carrying out analyses of the same sample
Integrated printer
Full connections (LAN - USB - Bluetooth barcode/QR code reader)
CDR WineLab® Jr
Partial analysis panel, customisable configuration
Up to 3 determinations simultaneously
Wireless connection to external printer
USB connections