Determination of Total Sulfur Dioxide (SO₂) in wine2

With CDR WineLab® is possible to determine the Total Sulfur Dioxide in wine and must in 1 minute with a simple photometric reading using the wine as is without having to resort to the distillation according with the reference method OIV or to the iodometric titration according with the simplified method. Calibration is not necessary.

This analysis can also be performed on wine vinegar.

Method

Test Principle

Chemical reaction involved in the analysis of total sulfur dioxide in wine and must

The sulfur dioxide contained in a wine is made to react with an organic disulphide in order to form a colored compound, whose intensity, measured at 420 nm in end-point, is directly proportional to the concentration of total sulfur dioxide in the sample.

Sulfur dioxide (SO2) is used in oenology mainly as preservative, but also for other purposes (for example for antiseptic, antioxidant, antioxidasic, solubilizing, combining and clarifying functions). Part of the SO2 that is added as potassium metabisulfite to musts and wines undergoes a series of reactions. Therefore, after a short period of time, most of the sulfur dioxide reacts with some of the substances present, binds with them and forms addition compounds (combined sulfur dioxide) that are unable to express the typical properties of unbound molecules. Therefore, only the part that does not combine (free sulfur dioxide) plays an important role in preserving wine from oxidizing alterations and from some micro-organisms. This test can be used to determine the total content of SO2 in wine, which is the sum of the free and combined sulfur dioxide fractions.

Calibration curve

The calibration curve of CDR WineLab®, obtained using reference methods.

Total Sulfur Analysis in wine and must: correlation curve with the reference method. R² = 0.978

Reagent test Kits

Measuring Range

AnalysesMeasuring rangeResolutionRepeatability

Analyzers for process and quality control in winemaking

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CDR WineLab®

  • Complete analysis panel, supplied already configured
  • Up to 16 determinations simultaneously
  • Possibility of carrying out analyses of the same sample 
  • Integrated printer
  • Full connections (LAN - USB - Bluetooth barcode/QR code reader)
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CDR WineLab® Junior

  • Partial analysis panel, supplied configured with 3 analyses of your choice, implementable
  • Up to 3 determinations simultaneously 
  • Wireless connection to external printer
  • USB connections
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