Technological methods for the rapid biophenol assay of olive oils

CDR OxiTester: the fast, simple and reliable olive oil testing system, to be used directly at theoil mill, in the oil processing plant or at the time of purchase

Polyphenols are among the most precious components of olive oil, and determine its typical fruity aroma, its tang, sharp taste, besides protecting the oil from decay and developing a powerful antioxidant action in the human organism. The actual concentration of polyphenols in the olive oil depends on the olive cultivar, on the harvesting period, the soil characteristics and the extraction process. The higher the concentration of polyphenols in olive oil, the better its organoleptic qualities, the more suited the production process, the longer the oil can last and have beneficial effects on human health.

The new Total Polyphenol (Biophenol) Test used by the CDR OxiTester has been tuned to the reference method COI/T.20/Doc. no. 29: 2009 by the Merceological Chemical Laboratory of Florence; the total olive oil polyphenol test results as obtained by the CDR OxiTester method are in good accord with the values obtained by the reference method.

Comparison of total polyphenol analysis results as performed by CDR OxiTester vs. reference method as per COI/T.20/Doc. n. 29: 2009, by the Merceological Chemistry Laboratory of Florence.

Below, the result of some test analyses performed on several extra virgin oil samples, using the new CDR method for the total polyphenol content assay.

Total polyphenol (biophenol) results

Advantages over the official method

The Merceological Chemical Laboratory of Florence has recognized several advantages of the analysis system contained in the CDR OxiTester versus the official method.

The new CDR FoodLab® method, used by the CDR OxiTester testing system, is a visible-light spectrometry method in which reducing substances react with a colored reagent, placed in pre-filled vials, thereby reducing their absorbance.

This analysis is extremely simple and fast (5 minutes), the reagent is ready inside the vial supplied to perform the analysis, and to which a fixed quantity of 10 μL of oil is added; after a short wait, the color of the vial is read by the CDR OxiTester analyzer.

The official method is, on the other hand, quite long and complicated, and requires sophisticated equipment and materials such as a high performance liquid chromatography (HPLC), several chemical substances of which some are dangerous, and assorted laboratory material.

This is because the official method approved by the International Olive Council is based on the extraction of minor polar biophenolic compounds directly from the olive oil by dissolving them in a methanol solution, followed by a quantitative assay using the HPLC with UV detection at a peak wavelength of 280 nm.

Click and discover all the analyses that you can perform yourself using the CDR OxiTester. Easy to use, in a few minutes results comparable to ISO methods

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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