During fermentation the symbiotic culture of bacteria and yeasts promotes the formation of acetic acid. The quantity of this acid can influence the organoleptic characteristics of the finished product. The possibility of its control during fermentation and in the finished product therefore constitutes an important quality parameter for this type of product.
CDR KombuchaLab: Instruments for the determination of Acetic Acid in Kombucha
The instruments are supplied pre-calibrated and ready to use.
The results are expressed according to the reference method.
The methods are simple: there is no need for an equipped laboratory or for specialist personnel.
 CDR KombuchaLab |
 CDR KombuchaLab Junior |
|
|---|---|---|
| Analyses | Kombucha: Alcohol by Volume Total Polyphenols Fermentable Sugars Acetic Acid Gluconic Acid Galacturonic Acid L-Malic Acid Total Lactic Acid Glycerol Yeast Assimilable Nitrogen (YAN) |
Configuration with tailored panel of analyses |
| Simultaneous Sample Analyses | 16 | 3 |
| Multiple Analyses on a Sample |  |
– |
Method
Test type: End Point.
Time testing: one test 6 minutes
Analysis sessions with multiple samples are possible for up to a maximum of 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior .
Calibration curve
The calibration curve of the CDR KombuchaLab instrument, obtained by comparing the CDR method with the enzymatic method for the determination of acetic acid, confirmed an excellent linearity of the system over the entire measurement range.
Test Principle
The acetic acid with enzymatic reaction is transformed into pyruvic acid which in turn reacts with NADH to give NAD+ and D-lactic acid.
The decrease in absorbance, measured at 366 nm (end-point), of the NADH solution is proportional to the quantity of acetic acid present in the sample.
Reagent test Kits
The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.
This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.
There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.
Code *300895 – Suitable for 100 tests
Code *300893 – Suitable for 10 tests
In case the method Acetic Acid [1.0 – 15.0] g/L is selected, use the dilution kits:
- Code *300911 – The kit allows 100 dilutions and contains 10 x code *300901.
- Code *300901 – The kit allows 10 dilutions.
Processing Sample
For precise and repeatable analysis, the sample must be degassed with the appropriate ultrasonic bath (code 222062) with the mini-centrifuge (code 222061) to separate the solid particles present in the solution.
Measuring Range
| Test | Measuring Range | Resolution | Repeatability |
|---|---|---|---|
| Acetic acid [0.05-1.2] g/L | 0.05 - 1.2 g/L Acetic acid | 0.01 g/L Acetic acid | 0.06 g/L Acetic acid |
| Acetic acid [1-15] g/l | 1 - 15 g/L | 0.1 g/L | 0.3 g/L |
