Determination of Alcohol Content in Kombucha

CDR KombuchaLab: the kombucha analysis system to optimize the production process obtaining results in real time.

The symbiotic culture of acetic bacteria and yeasts immersed in the sweetened tea infusion triggers an alcoholic fermentation that can bring about variable alcohol concentrations from traces by approximately one percentage point.
Normally the majority of Kombuchas on the market have an alcohol content that is below 0.5%.  The measurement of this parameter is very important for legal purposes as a concentration above 1.2% requires registration of the beverage produced as “alcoholic”.

CDR KombuchaLab: Instruments for the determination of Alcohol by Volume in Kombucha

The instruments are supplied pre-calibrated and ready to use.
The results are expressed according to the reference method.
The methods are simple: there is no need for an equipped laboratory or for specialist personnel.

  CDRKombuchaLab for analysis on Kombucha on pruction line
CDR KombuchaLab
CDRKombuchaLab Junior for analysis on Kombucha on pruction lineCDR KombuchaLab Junior
Analyses Kombucha:
Alcohol by Volume
Acetic Acid
Fermentable Sugars
Gluconic Acid
Total Lactic Acid
Yeast Assimilable Nitrogen (YAN)
Total Polyphenols
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si


Test type: End Point.
Time testing: one test 6 minutes.
Analysis sessions with multiple samples are possible for up to a maximum of 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior .

Correlation curve

The calibration curve of the CDR KombuchaLab  instrument, obtained by comparing the CDR method with the distillation method for determination of the alcohol content, confirmed an excellent linearity of the system over the entire measurement range.

Calibration curve of the CDR KombuchaLab instrument, obtained by comparing the CDR method with the distillation method for determination of the alcohol content in Kombucha. R²=0.9959.

Test Principle

Ethanol, in the presence of NAD+ , is oxidized by enzymatic route to acetaldehyde with the consequent production of NADH. The increase in absorbance, measured at 366 nm at the end-point, is directly proportional to the concentration of lactic acid in the sample. The result is expressed in ppm.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300665: 10 tests 

Code. *300666: 100 tests (10 packages of 10 tests)

When a dilution is requested, you have to use an appropriate reagent kits:

Code *300666: The kit allows 10 dilutions

Code *300667:- The kit allows 100 dilutions (10 packages of 10 tests)

The reagents have a shelf-life of 6 months

Processing Sample

For precise and repeatable analysis on the must, the sample must be degassed with the appropriate ultrasonic bath and centrifuged (code 222062) with the mini-centrifuge (code 222061) to separate the solid particles present in the solution. 

Measuring Range

Test Measuring range Resolution Accuracy
Alcoholic content 0.1 – 1.8% vol. 0.01% vol. 0.02% vol.

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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