Determination of Alcohol by Volume (ABV) in wine and must
Alcohol content is the measurement of ethanol content in an alcoholic beverage. In wine constitutes an important quality parameter.
The determination of alcohol by volume with CDR WineLab® is simpler and more rapid than OIV method, OIV methods: it consists in a simple photometric reading and operates on a small sample of wine as is without any distillation.
The analysis has a high repeatability and reliable. The results you obtain with CDR WineLab® are compliant with the results of OIV reference analytical methods.
Method
Sample treatment
If necessary (sparkling or fermenting wine), the sample shall be degassed using the specific ultrasonic bath (code 222062). In case of tests performed on must, the sample shall be centrifuged (code 222061) or filtered (code 300210). The analysis requests a rapid and simple dilution of the sample.
The test is suitable for sweet wines as well, because it is able to measure up to 17% v/v.
Principle of the test
Ethanol is oxidized in an alkaline environment through an enzymatic reaction and converted in acetic aldehyde in presence of NAD+, which in turns produces NADH. The increase of the absorbance, measured at 366 nm (end-point) is directly proportional to the concentration of ethanol in the sample.
The result is expressed in %v/v.
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Calibration curve
Alcohol by Volume (ABV) in the samples was determined using both the CDR WineLab® method and the distillation procedure specified by the OIV reference method MA-AS312-01A R2016 4.B. In its report, the Department of Chemistry 'Ugo Schiff' at the University of Florence confirmed that the two methods exhibited a high correlation coefficient.
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Reagent test Kits
Measuring range
Analyses | Measuring range | Resolution | Repeatability |
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Analyzers for process and quality control in winemaking
CDR WineLab®
Complete analysis panel, supplied already configured
Up to 16 determinations simultaneously
Possibility of carrying out analyses of the same sample
Integrated printer
Full connections (LAN - USB - Bluetooth barcode/QR code reader)
CDR WineLab® Jr
Partial analysis panel, customisable configuration
Up to 3 determinations simultaneously
Wireless connection to external printer
USB connections