Determination of Anthocyanins on grape

CDR WineLab® analysis system fast, simple to use and reliable for determining multiple parameters in wine and must

Anthocyanins are polyphenols present in the skin of grapes, whose colour changes according to the acidity of the surrounding environment. Anthocyanins are not very soluble in must and water, but very soluble in alcohol produced during fermentation. The colour of the future wine therefore depends on the degree of ripeness and on the duration of maceration of the skins. In addition, the concentration of polyphenols strongly influences the stability of a red wine during ageing. Therefore, in the case of a red wine, it is important to take into account the phenolic maturity of the grapes when choosing the harvest period.

The method considered to be the reference method for the determination of phenolic maturity (Glories method) is very time-consuming because it requires a long extraction and sample preparation phase. In addition, it is necessary that the analyses be carried out by an operator specialised in laboratory techniques.

The CDR WineLab® method for the determination of Anthocyanins in grapes and therefore of phenolic maturity optimizes the Glories method in order to make it even simpler, faster and more accurate.

The Analyzers CDR WineLab® for determining Anthocyanins on Grape

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR WineLab wine analyzerCDR WINELAB® CDR WineLab wine analyzer Junior VersionCDR WINELAB® JUNIOR
Analyses Wine and Must:
Alcohol by Volume
Acetic acid (volatile acidity)
Fermentable Sugars
Total Acidity
L-Lactic Acid
L-Malic Acid
Free sulfur dioxide
Total sulfur dioxide
Yeast Assimilable Nitrogen (YAN)
Copper
Catechins
Gluconic Acid
Acetaldehyde (Ethanal)
pH
Total Polyphenols Index
Glycerol
Colour (tonality and intensity)
Total Polyphenols (Folin Ciocalteu)
Anthocyanins on grapes
Total Anthocyanins
Polymerised Anthocyanins
HCl Index
Tannins
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Method

Test type: End Point.
Time testing: extraction 30 minutes – analysis 6 minutes.
Analysis sessions with several samples are possible, up to a maximum of 16 tests for CDR WineLab® and 3 tests for CDR WineLab Junior®.

Correlation curve

The correlation curve of the CDR WineLab® instrument, carried out in comparison to the Glories reference method, confirmed an excellent system linearity over the entire measuring range.

Correlation with the reference method (Glories Method) for the analysis of anthocyanins in grape. R²=0,9972

 

Test Principle

The total Anthocyanins present in the wine in must and grapes, in a very acidic environment and through the action of a concentrated SO2-based reagent, undergo a discoloration that is measured at 520 nm at end point. This discoloration is directly proportional to the concentration in the sample expressed in mg/L.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300459: 10 tests

Code *300336: 100 tests

The reagents have a shelf-life of 12 months

Sample Processing

Technique of extraction from grapes:

  1. Select a specific row from which to collect on both sides a total of 200-300 g of berries by sampling them as randomly as possible. Always use the selected row for the following samples in order to have a better representability of the sample.
  2. Grind the sample of grapes in the mixer for 1 minute.
  3. Take two samples, each of 6 g, and place them in the disposable containers provided for this purpose.
  4. Add 6 ml of solution A with pH 1 to the first sample.
  5. Add 6 ml of solution B with pH 3,2 to the second sample.
  6. Close the containers and wait 30 minutes. Mix them manually a couple of times.
  7. Centrifuge the suspensions. 0.5 mL of filtered sample is sufficient for the test.

Measuring Range

Test Measuring Range Resolution Repeatability
Anthocyanin grapes 10 - 1000 mg/L of Cyanidin 3-O-glucoside 1 mg/L of Cyanidin 3-O-glucoside 6 mg/L of Cyanidin 3-O-glucoside

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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