Determination of Total Anthocyanins in Wine and Must

CDR WineLab analysis system fast, simple to use and reliable for determining multiple parameters in wine and must

Anthocyanins, the pigments of the red grapes, are extracted during the grape maceration phase during alcoholic fermentation and then also in the case of possible post-fermentative maceration.

The CDR WineLab® method for the determination of anthocyanins was developed on the basis of the reference method (Ribéreau-Gayon and Stonestreet 1965).

The CDL WineLab® method is simpler to perform and the analysis time is considerably reduced compared to the reference method, which makes it possible to evaluate the concentration of anthocyanins in must or wine directly at the winery without the need to perform tests in a laboratory using specialist staff.

Determination of the total anthocyanins is part of a broad analysis panel developed by the CDR laboratories, implemented on CDR WineLab®, which offers the oenologist a precise and accurate control of both the polyphenolic extraction phase in red wines (maceration), and the subsequent polyphenol stabilisation phase (and therefore of colour stabilisation) that occurs with the micro-oxygenation process.

To learn more read ”Controlling Maceration and Micro-oxygenation in the wine-making process“.

The Analyzers CDR WineLab® for determining Total Anthocyanins in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR WineLab wine analyzerCDR WINELAB® CDR WineLab wine analyzer Junior VersionCDR WINELAB® JUNIOR
Analyses Wine and Must:

Alcohol by Volume
Acetic acid (volatile acidity)
Fermentable Sugars
Total Acidity
L-Lactic Acid
L-Malic Acid
Free sulfur dioxide
Total sulfur dioxide
Yeast Assimilable Nitrogen (YAN)
Gluconic Acid
Acetaldehyde (Ethanal)
pH
Total Polyphenols Index
Glycerol
Colour (tonality and intensity)
Total Polyphenols (Folin Ciocalteu)
Total Anthocyanins
Polymerised Anthocyanins
HCl Index
Tannins

Catechins
Copper
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Method

Test type: End Point.
Time testing: 11 minutes.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration curve

Correlation curve obtained with respect to the Ribéreau-Gayon and Stonestreet reference method of 1965

Correlation with the reference method for the analysis of anthocyanins in wine

 

Test Principle

Anthocyanins are the pigments of red grapes. They are found in the vacuolar juice of the skin cells and only exceptionally in specific vines in the pulp.
The analysis is based on the discolouration of anthocyanins in the presence of SO2. Analysis of the discolouration carried out by SO2 is measured at 520 nm in end-point and is proportional to the concentration of the anthocyanins present in the must/wine sample, expressed in mg/L.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *301025: 10 tests 

Code. *301020 : 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 6 months.

Sample Processing

Wine: Use the wine as it is after centrifugation.
Must: Use the must as it is after centrifugation and degassing.

Measuring Range

Test Measuring range Resolution Repeatability
Total Anthocyanins 50-1700 mg/L 0.1 mg/L 10 mg/L

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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