Glycerol is a substance produced by yeasts during alcoholic fermentation. In Kombucha this compound, based on its concentration, can determine a consistent effect on the organoleptic characteristics. The determination of its concentration in the finished product and above all its monitoring during fermentation constitute a fundamental aspect of quality control during the production process.
CDR KombuchaLab: Instruments for the determination of Glycerol in Kombucha
The instruments are supplied pre-calibrated and ready to use.
The results are expressed according to the reference method.
The methods are simple: there is no need for an equipped laboratory or for specialist personnel.
 CDR KombuchaLab |
 CDR KombuchaLab Junior |
|
|---|---|---|
| Analyses | Kombucha: Acetic Acid Alcohol by Volume Fermentable Sugars Gluconic Acid Total Lactic Acid Yeast Assimilable Nitrogen (YAN) Glycerol Total Polyphenols |
Configuration with tailored panel of analyses |
| Simultaneous Sample Analyses | 16 | 3 |
| Multiple Analyses on a Sample |  |
– |
Method
Test type: End Point.
Time testing: 3 minutes.
Are possible test sessions with several samples, up to 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior.
Test Principle
Glycerol, in the presence of an enzyme and a suitable chromogen, reacts causing the formation of a pink colour. Absorbance at a wavelength of 505 nm is proportional to the concentration of glycerol in the sample.
Calibration Curve
The calibration curve of the CDR KombuchaLab instrument, obtained by comparing the CDR method with the enzymatic method for the determination of glycerol, confirmed excellent linearity of the system over the entire measurement range.
Reagent test Kits
The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.
This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.
There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.
Code *300515 – The kit, designed for 10 tests
Code *300520 – The kit, designed for 100 tests
The reagents have a shelf-life of 12 months.
Processing of Sample Volume
For precise and repeatable analysis, the sample must be degassed with the appropriate ultrasonic bath (code 222062) with the mini-centrifuge (code 222061) to separate the solid particles present in the solution.
Measuring Range
| Analysis | Measuring range | Resolution | Repeatability |
|---|---|---|---|
| Glycerol [50 – 800] mg/L | 50 – 800 mg/L | 1 mg/L | 11 mg/L |
| Glycerol [700 – 5000] mg/L | 700 – 5000 mg/L | 1 mg/L | 100 mg/L |
