In addition to providing useful information on the quality of olive oil and on its degree of oxidation , this test enables to identify rectified oil added to virgin olive oil, by leveraging the fact that mechanically extracted oils do not contain double or triple conjugated bonds that typically form during rectification.
Rectified oils therefore exhibit high UV absorbance values, specifically in the 270 nm region, which are significantly higher as compared to those of virgin and extra virgin oils. The greater absorption in the 270 nm region, during the most advanced oxidation stages, is determined also by the formation of ketone groups.
Analyzers to test K270 in Olive Oil
You can determine the value of K270 in olive oil different systems of CDR FoodLab® range : CDR OxiTester for analysis of olive oil in oil mill, CDR FoodLab® for analysis of Vegetable and animal fats and oils
| CDR OXITESTER | CDR FOODLAB® | |
|---|---|---|
| Matrici alimentari | Olive Oil | Vegetable and animal fats and oils |
| Analisi | Free Fatty Acids (FFA) Peroxide Value (PV) Polyphenols/OSI K270 |
Free Fatty Acids (FFA) p-Anisidine Value Peroxide Value (PV) Soaps Polyphenols/OSI in olive oil Iodine Value (IV) in palm oil |
Method
End point test.
Test time: immediate.
It is possible to perform several test sessions with a maximum of 16 samples.
Principle of the test
The oil sample is dissolved in an appropriate solvent and the absorbance of the solution is read at 270 nm. Absorbance values are expressed as specific extinction E 1% 1 cm (1% extinction of a fatty substance in the prescribed solvent at a thickness of 1 cm), which is generally indicated as K and sometimes called extinction factor.
Calibration Curve / Correlation Data
The test calibration curve, obtained using reference methods, confirms the excellent linearity of the system in all the testing range.
Reagent test Kits
The reagents are packaged in 4 bottles of 25 mL + 100 empty cuvettes and 100 caps
Code. 300570: 100 tests.
The reagents have a shelf-life of 24 months.
Sample handling and usage of curves
Sample
Oil: collect as is (olive oil, seed oil, nut oil, etc). .
| Measuring range | Resolution | Repeatability |
|---|---|---|
| 0.020 – 1.570 | 0.001 | 0.01 |
