During fermentation the symbiotic culture of bacteria and yeasts promotes the formation of lactic acid of its D and L enantiomers, as well as other organic acids. The quantity of lactic acid can influence the organoleptic characteristics of the finished product. The possibility of its control during fermentation and in the finished product is therefore an important quality parameter for this type of beverage.
CDR KombuchaLab: Instruments for the determination of Total Lactic Acid in Kombucha
The instruments are supplied pre-calibrated and ready to use.
The results are expressed according to the reference method.
The methods are simple: there is no need for an equipped laboratory or for specialist personnel.
 CDR KombuchaLab |
 CDR KombuchaLab Junior |
|
|---|---|---|
| Analyses | Kombucha: Alcohol by Volume Acetic Acid Fermentable Sugars Gluconic Acid Total Lactic Acid Yeast Assimilable Nitrogen (YAN) Glycerol Total Polyphenols |
Configuration with tailored panel of analyses |
| Simultaneous Sample Analyses | 16 | 3 |
| Multiple Analyses on a Sample |  |
– |
Method
Test type: End Point.
Time testing: 11 minutes.
Are possible test sessions with several samples, up to 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior.
Test Principle
The D-lactic and L-lactic acids are oxidised by way of enzyme with pyruvic acid with the consequent production of NADH. The increase in absorbance, measured at 366 nm at the end-point, is directly proportional to the concentration of lactic acid in the sample. The result is expressed in ppm.
Calibration Curve
The calibration curve of the CDR KombuchaLab instrument, obtained by comparing the CDR method with the enzymatic method for the determination of total lactic acid (D/L), confirmed an excellent linearity of the system over the entire measurement range.
Reagent test Kits
The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.
This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.
There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.
Code *300715 – The kit, designed for 10 tests
Code *300720 – The kit, designed for 100 tests
The reagents have a shelf-life of 12 months.
Processing of Sample Volume
For precise and repeatable analysis, the sample must be degassed with the appropriate ultrasonic bath (code 222062) with the mini-centrifuge (code 222061) to separate the solid particles present in the solution.
Measuring Range
| Analysis | Measuring range | Resolution | Repeatability |
|---|---|---|---|
| Total lactic acid (D+L) | 50 – 3500 ppm | 1 ppm | 36 ppm |
