Determination of Total Lactic Acid in Kombucha

CDR KombuchaLab: the kombucha analysis system to optimize the production process obtaining results in real time.

During fermentation the symbiotic culture of bacteria and yeasts promotes the formation of lactic acid of its D and L enantiomers, as well as other organic acids. The quantity of lactic acid can influence the organoleptic characteristics of the finished product. The possibility of its control during fermentation and in the finished product is therefore an important quality parameter for this type of beverage.

CDR KombuchaLab: Instruments for the determination of Total Lactic Acid in Kombucha

The instruments are supplied pre-calibrated and ready to use.
The results are expressed according to the reference method.
The methods are simple: there is no need for an equipped laboratory or for specialist personnel.

  CDRKombuchaLab for analysis on Kombucha on pruction line
CDR KombuchaLab
CDRKombuchaLab Junior for analysis on Kombucha on pruction lineCDR KombuchaLab Junior
Analyses Kombucha:
Alcohol by Volume
Acetic Acid
Fermentable Sugars
Gluconic Acid
Total Lactic Acid
Yeast Assimilable Nitrogen (YAN)
Glycerol
Total Polyphenols
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Method

Test type: End Point.
Time testing:  11  minutes.
Are possible test sessions with several samples, up to 16 tests for CDR KombuchaLab and 3 tests for CDR KombuchaLab Junior.

Test Principle

The D-lactic and L-lactic acids are oxidised by way of enzyme with pyruvic acid with the consequent production of NADH. The increase in absorbance, measured at 366 nm at the end-point, is directly proportional to the concentration of lactic acid in the sample. The result is expressed in ppm.

Calibration Curve

The calibration curve of the CDR KombuchaLab  instrument, obtained by comparing the CDR method with the enzymatic method for the determination of total lactic acid (D/L), confirmed an excellent linearity of the system over the entire measurement range.

Calibration Curve of the CDR KonbuchaLab instrument, obtained by comparing the CDR method with the enzymatic method for the determination of D/L lactic acid in Kombucha. R²=0,9725.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300715 – The kit, designed for 10 tests

Code *300720 – The kit, designed for 100 tests

The reagents have a shelf-life of 12 months.

Processing of Sample Volume 

For precise and repeatable analysis, the sample must be degassed with the appropriate ultrasonic bath (code 222062) with the mini-centrifuge (code 222061) to separate the solid particles present in the solution.

Measuring Range

Analysis Measuring range Resolution Repeatability
Total lactic acid (D+L) 50 – 3500 ppm 1 ppm 36 ppm

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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