Determination of L-Lactic acid in Wine

CDR WineLab analysis system fast, simple to use and reliable for determining multiple parameters in wine and mustBy analyzing L-Malic acid and L-Lactic acid, it is possible to assess the correct progress of the malolactic fermentation.

The Analyzers CDR WineLab for determining L-Lactic Acid in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life of 12 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR WineLab wine analyzer Touch VersionCDR WINELAB TOUCH CDR WineLab wine analyzer Junior VersionCDR WINELAB JUNIOR
Analyses Fermentable Sugars
Total Acidity
Acetic Acid
L-Lactic Acid
L-Malic Acid
Malolactic Fermentation
Alcohol
Free sulfur dioxide (Free SO2)
Total sulfur dioxide (Total SO2)
pH
Yeast assimilable nitrogen (YAN)
Gluconic Acid
Glycerol
Polyphenols
TPI – Total Polyphenols Index
Acetaldehyde
Anthocyanins
Catechins
Colour intensity and tonality
Copper
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Method

Test type: End Point.
Time testing: one test 6 minutes – 50 Test/Hour.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration curve

The calibration curve of CDR WineLab, obtained using reference methods.

Lactic Acid in Wine Correlation with Reference Method

Test Principle

L-Lactic acid + NAD+ Analisi-AcidoLattico Pyruvate+ NADH
L-Lactic acid is oxidized in an alkaline environment through an enzymatic reaction and converted into pyruvate, which is turn produces NADH. The increase of absorbance, measured at 366 nm (end-point), is directly proportional to the concentration of L-Lactic acid in the sample.

Composition of the Kit and Reagents

Code *300280: The kit, designed for 10 tests, comprises:
1) R1: package with 10 pre-filled cuvettes with 1 mL of buffer at pH 9.
2) R1a: bottle with NAD+ in TRIS/HCl buffer.
3) R2: bottle with enzymatic solution (L-lactate-dehydrogenase).

Processing – Sample Volume – Measuring Range

Wine: use as is.

Test Measuring range
(g/L of lactic acid)
Sample volume Resolution
(g/L of lactic acid)
Repeatability
Lactic acid 0,05 -4,00 20 µL 0,01 0,05 g/L

Reagent developed by CDR S.r.l - ISO 9001 certified company

Click and Discover more about CDR WineLab: analysis system fast, simple to use and reliable for determining multiple parameters in wine and must

L-Lactic Acid was last modified: July 16th, 2016 by CDR s.r.l.