Determination of polymerised anthocyanins in wine and must

The CDR method for determining the degree of polymerisation of anthocyanins optimises the method developed by Parsec. The results of this analysis, together with those of the analysis of total anthocyanins, tannins and HCl index are used by the MoxEasy (Parsec) software to determine the optimal oxygen dose for micro-oxygenation.

The CDR WineLab^® method is used, in a simple and fast manner, to perform the analysis of polymerised anthocyans directly in the cellar. It improves the analytical repeatability of the analysis developed by Parsec as the standard deviation passes from 1.8 of the reference method to 0.5 of the CDR method.

The determination of the polymerised anthocyans is part of a broad analysis panel developed by the CDR laboratories, implemented on CDR WineLab^®, which offers the oenologist a precise and accurate control of both the polyphenolic extraction phase in red wines (maceration), and of the subsequent polyphenol stabilisation phase (and therefore of colour stabilisation) that occurs with the  micro-oxygenation process.

To learn more read ”Controlling Maceration and Micro-oxygenation in the wine-making process“.

The Analyzers CDR WineLab^® for determining Polymerised Anthocyanins in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

	CDR WINELAB®
Analyses	Wine and Must: Alcohol by Volume Acetic acid (volatile acidity) Fermentable Sugars Total Acidity L-Lactic Acid L-Malic Acid Free sulfur dioxide Total sulfur dioxide Yeast Assimilable Nitrogen (YAN) Galacturonic Acid Calcium Copper Catechins Gluconic Acid Acetaldehyde (Ethanal) pH Total Polyphenols Index Glycerol Colour (tonality and intensity) Total Polyphenols (Folin Ciocalteu) Anthocyanins Extraction on Grapes Total Anthocyanins Polymerised Anthocyanins HCl Index Tannins
Simultaneous Sample Analyses	16
Multiple Analyses on a Sample

Method

Test type: End Point.
Time testing: 11 minutes.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration curve

The correlation curve of the determination of anthocyanins polymerised in wine with CDR WineLab^®, carried out with respect to the Parsec method.

 

Test Principle

The method exploits the discolouration with SO₂. The method is based on the variation in absorbency of the wine at 420 and 520nm. The ratio between the sum of the two optical densities before and after the addition of sulphur dioxide estimates the concentration of the polymerised anthocyanins in the sample.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *301005: 10 tests 

Code. *301000 : 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 8 months.

Sample Processing

Wine: Use the wine as it is after centrifugation.
Must: Use the must as it is after centrifugation and degassing.

Measuring Range

Test	Measuring range	Resolution	Repeatability
Polymerised Anthocyanins	10 – 100%	0.1%	0.5%

Click and Discover more about CDR WineLab^®: analysis system fast, simple to use and reliable for determining multiple parameters in wine and must