Total Polyphenols test in olive oil

CDR OxiTester: the fast, simple and reliable olive oil testing system, to be used directly at theoil mill, in the oil processing plant or at the time of purchase

The antioxidant capacity of polyphenols plays an important role in the stability of olive oil, as there is a correlation between the amount of total polyphenols and resistance to oxidation over time.

The polyphenols have a protective action not only on oil but also on cells of the human body because they oppose the negative effects of free radicals.

The amount of polyphenols decreases during oil extraction so the test can be used to optimize the processing.

The oxidative stability index (OSI) of olive oil is directly proportional to the antioxidant capacity (polyphenols). It is calculated on the basis of the comparison between CDR method and AOCS official method Cd 12b-92 (Rancimat), performed by Minerva SA e Asteriadis SA in collaboration with the University of Athens, which estimates the time of induction, expressed in hours.

Analyzers to test polyphenols and Oil Stability Index (OSI) in Olive Oil 

CDR OxiTester: the fast, simple and reliable olive oil testing systemYou can determine the value of the polyphenols/OSI in olive oil with different systems of CDR FoodLab® range : CDR OxiTester for analysis of olive oil in oil mill, CDR FoodLab® for analysis of Vegetable and animal fats and oils

Analyses Olive Oil:
Peroxide Value
Acidity (FFA)
Total Polyphenols/OSI
Sugars in Olives
Oils and Fats:
Free Fatty Acids
Peroxide Value
Anisidine Value (AnV)
Soaps Test
Iodine Value test
Total Polyphenols/OSI

Polyphenols/OSI in olive oil
Iodine Value (IV) in palm oil


Testing time: 5 minutes – 30 Tests/Hour

It is possible to carry out several test sessions with a maximum of 16 samples.

Principle of the test

The polyphenols, in contact with a chromogenous compound in an alcoholic solution, are oxidized and decrease the color of the compound. The decreasing of color, read at 505 nm, is directly proportional to the concentration of polyphenols in the sample, expressed as mg / kg of tyrosol.

Calibration Curve / Correlation Data

The new Total Polyphenols (Biophenols) analysis used by the CDR OxiTester has been calibrated with the reference method IOC / T.20 / Doc. n. 29: 2009, by the Commodity Chemical Laboratory of Chamber of Commerce of Florence, Italy. They confirmed that the results of the Total Polyphenols of olive oil analysis, obtained with the CDR OxiTester, are compliant with the reference method.

CDR Oxitester method shows a very good correlation with OIC (International Olive Council) method.

Total Poliphenols test correlation CDR OxiTester Vs IOCofficialMethod

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300475: 100 tests

Code *300478: 10 tests

The reagents have a shelf-life of 12 months

Sample Handling

Use olive oil as is.

Measuring Range

Test Measuring Range Resolution Repeatability
Polyphenols - Stability index 200 - 1000 mg/Kg tyrosol 1 mg/Kg tyrosol 31 mg/Kg tyrosol
Polyphenols - Stability index 9 - 40 h (Induction Time) 0.1 h (Induction Time) 0.8 h (Induction Time)

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

Take a Look at CDR OxiTester olive oil testing in few minutes easy to use, compliance with AOCS methods

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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