Determination of Total Polyphenol Content in Wine

CDR WineLab analysis system fast, simple to use and reliable for determining multiple parameters in wine and mustPolyphenols are compounds contained in the grape peel and their presence in wine depends on the wine-making technique. Phenolic compounds produced by grapes play an important role in the characterization of different red wines, not only because they determine their color, but also because they significantly influence their fragrance and above all taste. The polyphenol content along with the “chromatic profile” of a wine provides very useful elements to asses the genuineness and quality of a wine.

The Analyzers CDR WineLab® for determining Polyphenols in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

 CDR WineLab wine analyzer CDR WINELAB®CDR WineLab wine analyzer Junior VersionCDR WINELAB® JUNIOR
AnalysesFermentable Sugars
Total Acidity
Acetic Acid
L-Lactic Acid
L-Malic Acid
Malolactic Fermentation
Alcohol
Free sulfur dioxide (Free SO2)
Total sulfur dioxide (Total SO2)
pH
Yeast assimilable nitrogen (YAN)
Gluconic Acid
Glycerol
Polyphenols
TPI – Total Polyphenols Index
Acetaldehyde (Ethanal)
Anthocyanins
Catechins
Colour intensity and tonality
Copper
Configuration with tailored panel of analyses
Simultaneous Sample Analyses163
Multiple Analyses on a Samplesi

Method

Test type: End Point.
Time testing: one test 5 minutes – 12 Test/Hour.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration curve

The calibration curve of CDR WineLab®, obtained using reference methods.

Total Polyphenol with CDR WineLab correlation with reference method

Test Principle

Polyphenols + Chromogen freccia Oxidized polyphenols + Reduced chromogen
Polyphenols are placed in contact with a colored complex in an alcoholic solution and oxidized, thus discoloring the complex. Discoloration, measured at 630 nm (end-point), is directly proportional to the concentration of polyphenols in the sample and expressed in mg/L of gallic acid.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300488: 10 tests 

Code. *300485: 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 12 months.

Processing – Sample Volume – Measuring Range

Wine: use as is.

TestMeasuring range
(mg/L of gallic acid)
Sample volumeResolutionRepeatability
Poyphenols in wine150 – 330010 µL0,01 mg/L10 mg/L/td>

For sample with values > 3300 mg/L, use half of the volume of the sample (5 µL) and multiply the result by 2.

Reagent developed by CDR S.r.l - ISO 9001 certified company

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Total Polyphenols was last modified: May 23rd, 2017 by CDR s.r.l.