Determination of Yeast Assimilable Nitrogen (YAN) in Must

CDR WineLab analysis system fast, simple to use and reliable for determining multiple parameters in wine and must

Yeast Assimilable Nitrogen (YAN) is a fundamental parameter to manage correctly the alcoholic fermentation. It is constituted by two components: the inorganic nitrogen present in must as ammonium ion (NH4+) and organic nitrogen constituted by a-amino nitrogen of amino acids.

The CDR WineLab® analytical method for the analysis in wine and must:

  • it is much easier than the method of the formol number generally used and it can be done in autonomous way in winery in real time without using toxic substances and with a simple photometric reading.
  • it allows the determination of the two nitrogen parameters separately, giving the possibility of making any corrections in a way more accurate than the analysis with formol number;
  • The result is not influenced by the presence of proline and hydroxy-proline, amino acids present in must, and not assimilated by yeasts.

Inorganic nitrogen (ammonium) is determined with the enzymatic way whereas organic nitrogen is determined with the o-phthalaldehyde method.

The Analyzers CDR WineLab® for Yeast Assimilable Nitrogen Test (YAN) in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR WineLab wine analyzer CDR WINELAB® CDR WineLab wine analyzer Junior VersionCDR WINELAB® JUNIOR
Analyses Wine and Must:

Alcohol by Volume
Acetic acid (volatile acidity)
Fermentable Sugars
Total Acidity
L-Lactic Acid
L-Malic Acid
Free sulfur dioxide
Total sulfur dioxide
Yeast Assimilable Nitrogen (YAN)
Gluconic Acid
Acetaldehyde (Ethanal)
pH
Total Polyphenols Index
Glycerol
Colour (tonality and intensity)
Total Polyphenols
Total Anthocyanins
Polymerised Anthocyanins
HCl Index
Tannins

Catechins
Copper
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Inorganic Nitrogen

Method

Test type: End Point.
Time Testing: one test 4 minutes.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration Curve

The calibration curve of CDR WineLab® for the analysis of Yeast Assimilable Nitrogen Inorganic in must, obtained using reference methods, confirms the excellent linearity of the system over the whole testing range.

Inorganic Nitrogen Concentration correlation with CDR WineLab Method

 

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300435: 10 tests 

Code. *300430: 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 6 months

Test Principle

a-cheto glutarate + NADH + NH4+YAN -Inorganic-reaction Acid L-glutammic + NAD+ +H2O

The ammonium reacts by enzimatic path-way with NADH and a-ketoglutarate to give NAD+ and glutamate. The decrease in absorbance of the solution of NADH, measured at 366 nm (end-point), is proportional to the amount of ammonium nitrogen in the sample.

Organic Nitrogen

Method

Test type: End Point.
Time testing: one test 4 minutes.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration Curve

The calibration curve of CDR WineLab® for the analysis of Yeast Assimilable Nitrogen Organic in must, obtained using reference methods, confirms the excellent linearity of the system over the whole testing range.

Organic Nitrogen Concentration with CDR WineLab Method

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300435: 10 tests 

Code. *300430: 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 6 months

Test Principle

YAN -Organic-reaction

The a-amino nitrogen reacts, in presence of N-acetyl-L-cysteine, with the o-phthalaldehyde to form a isoindolic derivative. The difference in absorbance, measured at 366 nm (end-point) is correlated with the amount of organic nitrogen expressed as mg / L of N.

Analyses of yeast assimilable nitrogen (YAN) for optimum management of alcoholic fermentation

Download VINIFICATION IN THE ERA OF GLOBAL WARMING  Check out why, especially in hot and dry vintages, YAN analysis is a fundamental tool for better management of the alcoholic fermentation, allowing for prudent planning of yeast nutrition and thus avoiding dangerous stucks fermentation. Download the paper: VINIFICATION IN THE ERA OF GLOBAL WARMING

Processing – Sample Volume

If necessary, the sample shall be degassed using the specific ultrasonic bath. In case of tests performed on must, the sample shall be first centrifuged.

Measuring Range

Test Measuring range Resolution Repeatability<
Inorganic Nitrogen 30 – 300
mg/L di N inorganic
1 2 mg/L
Organic Nitrogen 30 – 300
mg/L di N organic
1 2 mg/L

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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