Determination of Yeast Assimilable Nitrogen (YAN) in Must

CDR WineLab analysis system fast, simple to use and reliable for determining multiple parameters in wine and mustYeast Assimilable Nitrogen (YAN) is a fundamental parameter to manage correctly the alcoholic fermentation. It is constituted by two components: the inorganic nitrogen present in must as ammonium ion (NH4+) and organic nitrogen constituted by a-amino nitrogen of amino acids.

The CDR WineLab® analytical method for the analysis in wine and must:

  • it is much easier than the method of the formol number generally used and it can be done in autonomous way in winery in real time without using toxic substances and with a simple photometric reading.
  • it allows the determination of the two nitrogen parameters separately, giving the possibility of making any corrections in a way more accurate than the analysis with formol number;
  • The result is not influenced by the presence of proline and hydroxy-proline, amino acids present in must, and not assimilated by yeasts.

Inorganic nitrogen (ammonium) is determined with the enzymatic way whereas organic nitrogen is determined with the o-phthalaldehyde method.

The Analyzers CDR WineLab® for determining Yeast Assimilable Nitrogen (YAN) in Wine and Must

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
It is also possible to standardize the system using samples with a known titration.
The reagents have a shelf-life untill 18 months
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR WineLab wine analyzer CDR WINELAB® CDR WineLab wine analyzer Junior VersionCDR WINELAB® JUNIOR
Analyses Fermentable Sugars
Total Acidity
Acetic Acid
L-Lactic Acid
L-Malic Acid
Malolactic Fermentation
Alcohol
Free sulfur dioxide (Free SO2)
Total sulfur dioxide (Total SO2)
pH
Yeast assimilable nitrogen (YAN)
Gluconic Acid
Glycerol
Polyphenols
TPI – Total Polyphenols Index
Acetaldehyde (Ethanal)
Anthocyanins
Catechins
Colour intensity and tonality
Copper
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Inorganic Nitrogen

Method

Test type: End Point.
Time Testing: one test 4 minutes 60 Test/Hour.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration Curve

The calibration curve of CDR WineLab® for the analysis of Yeast Assimilable Nitrogen Inorganic in must, obtained using reference methods, confirms the excellent linearity of the system over the whole testing range.

Inorganic Nitrogen Concentration correlation with CDR WineLab Method

 

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300435: 10 tests 

Code. *300430: 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 6 months

Test Principle

a-cheto glutarate + NADH + NH4+YAN -Inorganic-reaction Acid L-glutammic + NAD+ +H2O

The ammonium reacts by enzimatic path-way with NADH and a-ketoglutarate to give NAD+ and glutamate. The decrease in absorbance of the solution of NADH, measured at 366 nm (end-point), is proportional to the amount of ammonium nitrogen in the sample.

Organic Nitrogen

Method

Test type: End Point.
Time testing: one test 4 minutes 60 Test/Hour.
Are possible test sessions with several samples, up to a maximum of 16.

Calibration Curve

The calibration curve of CDR WineLab® for the analysis of Yeast Assimilable Nitrogen Organic in must, obtained using reference methods, confirms the excellent linearity of the system over the whole testing range.

Organic Nitrogen Concentration with CDR WineLab Method

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code. *300435: 10 tests 

Code. *300430: 100 tests (10 packages of 10 tests)

The reagents have a shelf-life of 6 months

Test Principle

YAN -Organic-reaction

The a-amino nitrogen reacts, in presence of N-acetyl-L-cysteine, with the o-phthalaldehyde to form a isoindolic derivative. The difference in absorbance, measured at 366 nm (end-point) is correlated with the amount of organic nitrogen expressed as mg / L of N.

Processing – Sample Volume – Measuring Range

 

Test Measuring range Sample volume Resolution Repeatability
Inorganic Nitrogen 30 – 300
mg/L di N inorganic
10 µL of wine 1 2 mg/L
Organic Nitrogen 30 – 300
mg/L di N organic
20 µL of wine 1 2 mg/L

Reagent developed by CDR S.r.l - ISO 9001 certified company

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Yeast Assimilable Nitrogen (YAN) was last modified: June 30th, 2017 by CDR s.r.l.