Determination of FAN in beer and wort

Free Amino Nitrogen by OPA (FAN) is a test that allows to manage correctly the alcohol fermentation. In fact the lack of Nitrogen can cause a slowing-down or even the stop of the fermentation, compromising seriously the quality and the organoleptic characteristics of the beer.

FAN by OPA analysis with the CDR BeerLab® method allows to determine the concentration of the two components separately, giving the possibility to carry out with accuracy the appropriate corrections before and during the alcohol fermentation. The kit for the Inorganic Nitrogen is selective for the ammonium ion and allows to adjust with accuracy a possible addition of sulfate/hydrogen-ammonium phosphate in the wort.

Method

Test Principle

The α-amino Nitrogen reacts in presence of N-acetyl-L-cysteine with o-phthalaldehyde forming an isoindole derived. The difference of absorbance, measured at 366 nm (end point), is correlated with the quantity of Organic Nitrogen expressed in mg/L of Nitrogen.

Calibration Curve

The calibration curve was made using samples with known values. The trend of the curve confirms the excellent linearity of the system over the entire measurement range.

The calibration curve of the determination of free amino nitrogen in wort and beer was made using known samples. R² = 0.9962

Reagent test Kits

Measuring range

AnalysesMeasuring rangeResolutionRepeatability

The Analyzers for process and quality control in beer brewing

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CDR BeerLab®

  • Complete analysis panel, supplied already configured
  • Up to 16 determinations simultaneously
  • Possibility of carrying out analyses of the same sample
  • Integrated printer
  • Full connections (LAN - USB - Bluetooth barcode/QR code reader)
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CDR BeerLab® Jr

  • Partial analysis panel, supplied configured with 3 analyses of your choice, implementable
  • Up to 3 determinations simultaneously
  • Wireless connection to external printer
  • USB connections