CDR FoodLab® is the chemical analyzer for quality and process control that allows you to determine a wide range of parameters of many foods, animal feeds and pet foods.

D-3-hydroxybutyric acid test in Egg and Egg Products

The N.853/2004 REGULATION (CE) states that the level of D-3-hydroxybutyric acid in egg products should not exceed 10mg/kg of dry matter

CDR FoodLab® is an innovative analysis system which simplifies and speeds up the procedures traditionally used allowing to determine the D-3-hydroxybutyric acid quickly: 5 minutes for sample preparation, testing time 5 minutes.


Test principle

The pasteurization of egg products is based on the use of not high temperatures, because egg proteins are sensitivity to heat. For this reason, the use of bad eggs, increases the health risk and reduces the shelf-life of the fresh egg products. The use of incubated or decayed eggs can be detected verifying the presence of some organic acids indicative of metabolic products and microbial decomposition such as succinic acid, lactic acid and D-3-hydroxybutyric acid. The best method currently highlighting this fraud is the test of D-3-hydroxybutyric acid, very low in fresh eggs (<1mg/kg), but much more high in the wastes of incubator. The analysis is generally carried out by extraction and gas chromatography.

D-3-hydroxybutyric + NAD+ -----→ Pink complex

The D-3-hydroxybutyric acid reacts, by an enzymatic reaction with the NAD+ and induces the formation of a pink color whose intensity, measured at 505 nm, is directly proportional to the concentration of D-3-hydroxybutyric acid in the sample. The result is expressed as ppm of D-3-hydroxybutyric acid.

Correlation Data

Comparative tests on samples of mixed eggs where was add a D-3-hydroxybutyric acid standard, checked with CDR FoodLab® method, confirmed a very good alignment.

Comparative tests on samples of mixed eggs where was add a D-3-hydroxybutyric acid standard. R²=0.9928

Reagent test Kits

Measuring range

AnalysesMeasuring rangeResolutionRepeatability

The Analyzers for quality control of egg products


CDR FoodLab®

  • Complete analysis panel, supplied already configured
  • Up to 16 determinations simultaneously
  • Possibility of carrying out analyses of the same sample
  • Integrated printer
  • Full connections (LAN - USB - Bluetooth barcode/QR code reader)

CDR FoodLab® JR

  • Partial analysis panel, supplied configured with 3 analyses of your choice, implementable
  • Up to 3 determinations simultaneously
  • Wireless connection to external printer
  • USB connections