Peroxidase test in milk
The persistence of lacto-peroxidase activity in pasteurized milk provides a good indication of the quality of a product, because only a raw milk of good microbiological quality can be put through a mild pasteurization process in order to not inactivate this enzyme. Traditional methods are often qualitative (present/not present) and thus able only to indicate whether the thermal process has been performed.
Quantifying lacto-peroxidase in pasteurized milk enables instead to determine the nutritional quality of milk. Higher value of peroxidase means that the milk has preserved its original characteristics.
Method
Test type: End Point.
Testing time: 10 minutes.
Analysis sessions with multiple samples are possible up to a maximum of 16 tests for CDR FoodLab® and 3 tests for CDR FoodLab® Junior.
Sample treatment
Use milk as is.
Principle of the test
In presence of an indicator and hydrogen peroxide, milk peroxidase catalyzes forming a red compound with an intensity that is directly proportional to the concentration of peroxidase in the sample when measured at 505 nm. CDR’s innovative method enables to easily and quickly quantify lacto-peroxidase in milk.
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Calibration curve
The quantitative determination of peroxidase is an innovative test and it is therefore not easy to find samples that can be used as reference or official reference methods. Thus, CDR’s laboratories carried out several test, using CDR FoodLab®’s method, on samples of different manufacturers and compared the resulting values with those specified in the technical data sheet of the Reflectoquant® reflectometric method. The results compared well.
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Reagent test Kits
Measuring range
| Analyses | Measuring range | Resolution | Repeatability |
|---|---|---|---|
| POD - Serum proteins | 100 - 8000 U/L Peroxidase | 1 U/L Peroxidase | 172 U/L Peroxidase |
Analyzers for quality control of milk and dairy products
CDR FoodLab®
Complete analysis panel, supplied already configured
Up to 16 determinations simultaneously
Possibility of carrying out analyses of the same sample
Integrated printer
Full connections (LAN - USB - Bluetooth barcode/QR code reader)
CDR FoodLab® Jr
Partial analysis panel, customisable configuration
Up to 3 determinations simultaneously
Wireless connection to external printer
USB connections
