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Alkaline phosphatase (ALP) in milk

The chemical analysis of the milk and dairy products for quality control for every stage of the production chain with CDR FoodLabThe alkaline phosphatase (ALP) is an enzyme normally present in raw milk and it is inactivated in conditions of heat treatment. The temperature of inactivation of ALP is slightly higher than that required for the destruction of pathogenic bacteria. So the ALP test in pasteurized milk is used to verify if the heating process of pasteurization is done correctly.

Analyzers for determining Alkaline phosphatase (ALP) in milk

 

  CDR FoodLab for performing a range of tests in milk and a variety of dairy products CDR FOODLAB® CDR FoodLab Junior for performing a range of tests in milk and a variety of dairy products CDR FOODLAB® JUNIOR
Analyses Milk and Dairy Products:
Lactose
L-Lactic acid
Milk Urea Nitrogen (MUN)
Alkaline phosphatase (ALP)
Ammonia
Chloride
Hydrogen peroxide
ε-fructosyl-lisine (Furosine)
Peroxidase
 Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si –

Features

It is not necessary skilled staff nor a laboratory for the analysis.
Results are correlated with reference method results.
It is free of service and maintenance cost.
With the same analyzers you can also perform analysis of egg, tomato, vegetable purees, cheeses and fats.

Method

Test type End Point
Test time: 30 minutes
It is possible to carry out several test sessions with a maximum of 16 samples.

Test principle

p-Nitrofenilfosfato + OH- reazione-alp yellow complex

Alkaline phosphatase induces the hydrolysis of p-nitrophenyl phosphate in an alkaline solution, and forms a yellow colored complex whose intensity, measured at 405 nm, is directly proportional to the concentration of ALP in the sample.

Calibration curve

The calibration curve was performed using various additions of raw milk in pasteurized milk. The values reported are correlated with those reported in the literature (“ALP testing for milk Pasteurization” Cornell University – Dairy science facts 1998).

alp test in milk CDR FoodLab vs Theoretical value

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300225: 100 tests

Code *300228: 10 tests

The reagents have a shelf-life of 12 months

Sample Handling 

Sample
Use pasteurized cow milk.

Measuring range

Test Measuring Range Resolution Repeatability
ALP 0.1 - 7 U/L 0.01 U/L 0.12 U/L

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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