Alkaline phosphatase (ALP) in milk

The chemical analysis of the milk and dairy products for quality control for every stage of the production chain with CDR FoodLabThe alkaline phosphatase (ALP) is an enzyme normally present in raw milk and it is inactivated in conditions of heat treatment. The temperature of inactivation of ALP is slightly higher than that required for the destruction of pathogenic bacteria. So the ALP test in pasteurized milk is used to verify if the heating process of pasteurization is done correctly.

Analyzers for determining Alkaline phosphatase (ALP) in milk and dairy products

 

  CDR FoodLab for performing a range of tests in milk and a variety of dairy products CDR FOODLAB® CDR FoodLab Junior for performing a range of tests in milk and a variety of dairy products CDR FOODLAB® JUNIOR
Analyses e-Fructosyl Lisine
L-Lactic Acid
Ammonia
Chloride
Alkaline phosphatase (ALP)
Peroxidase
Hydrogen peroxide
Milk Urea Nitrogen (MUN)
Lactose
Peroxide value (PV)
Free Fatty Acids (FFA)
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

It is not necessary skilled staff nor a laboratory for the analysis.
Results are correlated with reference method results.
It is free of service and maintenance cost.
With the same analyzers you can also perform analysis of egg, tomato, vegetable purees, cheeses and fats.

Method

Test type End Point
Test time: 30 minutes
It is possible to carry out several test sessions with a maximum of 16 samples.

Test principle

p-Nitrofenilfosfato + OH- reazione-alp yellow complex

Alkaline phosphatase induces the hydrolysis of p-nitrophenyl phosphate in an alkaline solution, and forms a yellow colored complex whose intensity, measured at 405 nm, is directly proportional to the concentration of ALP in the sample.

Calibration curve

The calibration curve was performed using various additions of raw milk in pasteurized milk. The values reported are correlated with those reported in the literature (“ALP testing for milk Pasteurization” Cornell University – Dairy science facts 1998).

alp test in milk CDR FoodLab vs Theoretical value

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Reagent test kit *300225 suitable for 100 tests.

Reagent test kit *300228 suitable for 10 tests.

The reagents have a shelf-life of 12 months.

Sample Handling 

Sample
Use pasteurized cow milk.

Measuring range

Measuring range
(U/L fosfatasi alcalina)
Sample volume Resolution
(U/L fosfatasi alcalina)
Repeatability
0,1 – 7 150 µL 0,1 0,1 U/L

Reagent developed by CDR S.r.l - ISO 9001 certified company

 

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Alkaline phosphatase (ALP) was last modified: May 23rd, 2017 by CDR s.r.l.