Alkaline phosphatase (ALP) in milk
The alkaline phosphatase (ALP) is an enzyme normally present in raw milk and it is inactivated in conditions of heat treatment. The temperature of inactivation of ALP is slightly higher than that required for the destruction of pathogenic bacteria. So the ALP test in pasteurized milk is used to verify if the heating process of pasteurization is done correctly.
Analyzers for determining Alkaline phosphatase (ALP) in milk and dairy products
 CDR FOODLAB® |
 CDR FOODLAB® JUNIOR |
|
|---|---|---|
| Analyses | e-Fructosyl Lisine L-Lactic Acid Ammonia Chloride Alkaline phosphatase (ALP) Peroxidase Hydrogen peroxide Milk Urea Nitrogen (MUN) Lactose Peroxide value (PV) Free Fatty Acids (FFA) |
Configuration with tailored panel of analyses |
| Simultaneous Sample Analyses | 16 | 3 |
| Multiple Analyses on a Sample |  |
– |
It is not necessary skilled staff nor a laboratory for the analysis.
Results are correlated with reference method results.
It is free of service and maintenance cost.
With the same analyzers you can also perform analysis of egg, tomato, vegetable purees, cheeses and fats.
Method
Test type End Point
Test time: 30 minutes
It is possible to carry out several test sessions with a maximum of 16 samples.
Test principle
p-Nitrofenilfosfato + OH- 
yellow complex
Alkaline phosphatase induces the hydrolysis of p-nitrophenyl phosphate in an alkaline solution, and forms a yellow colored complex whose intensity, measured at 405 nm, is directly proportional to the concentration of ALP in the sample.
Calibration curve
The calibration curve was performed using various additions of raw milk in pasteurized milk. The values reported are correlated with those reported in the literature (“ALP testing for milk Pasteurization” Cornell University – Dairy science facts 1998).
Reagent test Kits
The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.
This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.
There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.
Reagent test kit *300225 suitable for 100 tests.
Reagent test kit *300228 suitable for 10 tests.
The reagents have a shelf-life of 12 months.
Sample Handling
Sample
Use pasteurized cow milk.
Measuring range
| Measuring range (U/L fosfatasi alcalina) |
Sample volume | Resolution (U/L fosfatasi alcalina) |
Repeatability |
|---|---|---|---|
| 0,1 – 7 | 150 µL | 0,1 | 0,1 U/L |
