Determination of FAN in beer and wort
Free Amino Nitrogen by OPA (FAN) is a test that allows to manage correctly the alcohol fermentation. Infact, the lack of Nitrogen can cause a slowing-down or even the stop of the fermentation, compromising seriously the quality and the organoleptic characteristics of the beer.
FAN analysis with the CDR BeerLab® method gives the possibility to carry out with accuracy the appropriate corrections before and during the alcohol fermentation.
Method
Sample treatment
Use on beer or wort. The beer sample needs to be degassed using the ultrasonic bath (code 222062) before taking the sample. For higher accuracy in the analysis of wort we recommend to centrifuge (code 222061) the sample.
Principle of the test
Organic Nitrogen - The α-amino Nitrogen reacts in presence of N-acetyl-L-cysteine with o-phthalaldehyde forming an isoindole derived. The difference of absorbance, measured at 366 nm (end point), is correlated with the quantity of Organic Nitrogen expressed in mg/L of Nitrogen.
Calibration curve
Calibration curve obtained with samples of known values.
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Reagent test Kits
Code*300785: 10 tests
Measuring range
| Analyses | Measuring range | Resolution | Repeatability |
|---|---|---|---|
| Organic nitrogen (FAN) beer [10-150]mg/L | 10 - 150 mg/L | 1 mg/L | 4 mg/L |
| Organic nitrogen (FAN) wort [80-500]mg/L | 80 - 500 mg/L | 1 mg/L | 9 mg/L |
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The Analyzers for process and quality control in beer brewing
CDR BeerLab®
Complete analysis panel, supplied already configured
Up to 16 determinations simultaneously
Possibility of carrying out analyses of the same sample
Integrated printer
Full connections (LAN - USB - Bluetooth barcode/QR code reader)
CDR BeerLab® Jr
Partial analysis panel, customisable configuration
Up to 3 determinations simultaneously
Wireless connection to external printer
USB connections