Determination of FAN in beer and wort

CDR BeerLab is the easy, fast and reliable testing system to be used directly at the brewery with no need of expertise in chemical analysis
Free Amino Nitrogen by OPA (FAN) is a test that allows to manage correctly the alcohol fermentation. In fact the lack of Nitrogen can cause a slowing-down or even the stop of the fermentation, compromising seriously the quality and the organoleptic characteristics of the beer.

FAN by OPA analysis with the CDR BeerLab® method allows to determine the concentration of the two components separately, giving the possibility to carry out with accuracy the appropriate corrections before and during the alcohol fermentation. The kit for the Inorganic Nitrogen is selective for the ammonium ion and allows to adjust with accuracy a possible addition of sulfate/hydrogen-ammonium phosphate in the wort.

The systems to test Free Amino Nitrogen by OPA (FAN) in beer and wort

Free Amino Nitrogen by OPA (FAN) can be measured using CDR BeerLab®, a system which enables you to carry out a wide panel of chemical analyses on beer and water, using a single instrument, in a process that is faster and simpler than traditional methods.
With CDR BeerLab® brewers and breweries of any size can independently carry out brewing process controls without having to rely on external laboratories.

  CDR BeerLab: chemical analysis system specifically designed to monitor the brewing process  in breweryCDR BEERLAB® CDR BeerLab Junior: chemical analysis system specifically designed to monitor the brewing process  in breweryCDR BEERLAB® JUNIOR
Analyses

Beer and Wort:
Fermentable Sugars
Lactic Acid (D + L)
Bitterness
Color
Alcohol Content
pH
Starch
Free Aminio Nitrogen by OPA (FAN)
Total sulfur dioxide (SO2)
Vicinal diketones (VDKs)
Acetic Acid

Water:
Calcium
Magnesium
Bicarbonate
Chloride
Potassium
Sulfate
Zinc

Configuration with tailored panel of analyses

Beer and Wort:
Fermentable Sugars
Lactic Acid (D + L)
Bitterness
Color
Alcohol Content
pH
Starch
Free Aminio Nitrogen by OPA (FAN)
Total sulfur dioxide (SO2)
Vicinal diketones (VDKs)
Acetic Acid

Water:
Calcium
Magnesium
Bicarbonate
Chloride
Potassium
Sulfate
Zinc

Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Method

Test type: End Point.
Time testing: 4 minutes.
Are possible test sessions with several samples, up to 16 tests for CDR BeerLab® and 3 tests for CDR BeerLab® Junior.

Calibration Curve

Calibration curve obtained with samples of known values.

Calibration curve of Free Amino Nitrogen by OPA FAN analysis in wort perfomed by CDR BeerLab

Test Principle

The α-amino Nitrogen reacts in presence of N-acetyl-L-cysteine with o-phthalaldehyde forming an isoindole derived. The difference of absorbance, measured at 366 nm (end point), is correlated with the quantity of Organic Nitrogen expressed in mg/L of Nitrogen.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

  • Code *300785 – The kit, designed for 10 tests
  • Code *300780 – The kit, designed for 100 tests 

The reagents have a shelf-life of 6 months.

Processing of Sample

Use on wort. For higher accuracy in the analysis of wort we recommend to centrifuge the sample.

Measuring Range

Analysis Measuring range Sample volume Resolution Repeatability
FAN by OPA 30 – 300 ppm 20 μL 1 ppm 2 ppm

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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Free Amino Nitrogen by OPA (FAN) was last modified: March 16th, 2018 by CDR s.r.l.