Determination of FAN in beer and wort

CDR BeerLab® is the easy, fast and reliable testing system to be used directly at the brewery with no need of expertise in chemical analysis
Free Amino Nitrogen by OPA (FAN) is a test that allows to manage correctly the alcohol fermentation. In fact the lack of Nitrogen can cause a slowing-down or even the stop of the fermentation, compromising seriously the quality and the organoleptic characteristics of the beer.

FAN by OPA analysis with the CDR BeerLab® method allows to determine the concentration of the two components separately, giving the possibility to carry out with accuracy the appropriate corrections before and during the alcohol fermentation. The kit for the Inorganic Nitrogen is selective for the ammonium ion and allows to adjust with accuracy a possible addition of sulfate/hydrogen-ammonium phosphate in the wort.

The systems to test Free Amino Nitrogen by OPA (FAN) in beer and wort

Free Amino Nitrogen by OPA (FAN) can be measured using CDR BeerLab®, a system which enables you to carry out a wide panel of chemical analyses on beer and water, using a single instrument, in a process that is faster and simpler than traditional methods.
With CDR BeerLab® brewers and breweries of any size can independently carry out brewing process controls without having to rely on external laboratories.

  CDR BeerLab: chemical analysis system specifically designed to monitor the brewing process  in breweryCDR BEERLAB® CDR BeerLab Junior: chemical analysis system specifically designed to monitor the brewing process  in breweryCDR BEERLAB® JUNIOR
Analyses Beer, Wort and Water:
Bitterness (IBU)
Fermentable Sugars
Alcohol by Volume (ABV)
Acetic acid
Yeast Vitality
Vicinal Diketons (VDKs)
Total sulfur dioxide
Free Amino Nitrogen (FAN) by OPA
Lactic Acid (D + L)
Polyphenols in beer
Carbohydrates in Beer
Calcium in Beer and Water
Alkalinity of water
Bicarbonate in Water
Zinc in Water
Sulfate in Water
Magnesium in Water
Potassium in Water
Chloride in Water

Configuration with tailored panel of analyses

Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si


Test type: End Point.
Time testing: 4 minutes.
Are possible test sessions with several samples, up to 16 tests for CDR BeerLab® and 3 tests for CDR BeerLab® Junior.

Calibration Curve

Calibration curve obtained with samples of known values.

Calibration curve of Free Amino Nitrogen by OPA FAN analysis in wort perfomed by CDR BeerLab

Test Principle

The α-amino Nitrogen reacts in presence of N-acetyl-L-cysteine with o-phthalaldehyde forming an isoindole derived. The difference of absorbance, measured at 366 nm (end point), is correlated with the quantity of Organic Nitrogen expressed in mg/L of Nitrogen.


Analysis of Mashing in the Brewery

Download the case study "Analysis of Mashing in the Brewery" wiritten by Dr Lee Walsh, QCL, CDR BeerLab distributor in UKIs it possible to reduce the mashing time improving the process efficiency with in-house brewing process control? The case study shows that it is possible to reduce the mashing time improving the process efficiency using CDR BeerLab® to perform chemical analysis of beer for in-house brewering process control.

Download the case study “Analysis of Mashing in the Brewery” wiritten by Dr Lee Walsh, QCL, CDR BeerLab® distributor in UK

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

  • Code *300785 – The kit, designed for 10 tests
  • Code *300780 – The kit, designed for 100 tests 

The reagents have a shelf-life of 6 months.

Processing of Sample

Use on wort. For higher accuracy in the analysis of wort we recommend to centrifuge the sample.

Measuring Range

Analysis Measuring range Resolution Repeatability
FAN by OPA 30 – 300 ppm 1 ppm 2 ppm

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
Find out how it works