D-3-hydroxybutyric acid test in Egg and Egg Products

Simple chemical analysis of egg products and eggs thanks to CDR FoodLab® with very easy and quick sample preparationThe N.853/2004 REGULATION (CE) states that the level of D-3-hydroxybutyric acid in egg products should not exceed 10mg/kg of dry matter

CDR FoodLab® is an innovative analysis system which simplifies and speeds up the procedures traditionally used allowing to determine the D-3-hydroxybutyric acid quickly: 5 minutes for sample preparation, testing time 5 minutes

Systems to test D-3-hydroxybutyric acid in eggs products and eggs

  CDR FoodLab® analyzer for tests on egg products and eggsCDR FOODLAB® CDR FoodLab® Junior analyzer for tests on egg products and eggsCDR FOODLAB® JUNIOR
Analyses Egg products:
Lactic Acid
D-3-hydroxybutyric acid
Colour (Beta-Carotene)
Egg products:
Lactic Acid
D-3-hydroxybutyric acid
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si


Test type: kinetic
Test time: 5 minutes
It is possible to carry out several test sessions with a maximum of 16 samples.

Test principle

The pasteurization of egg products is based on the use of not high temperatures, because egg proteins are sensitivity to heat. For this reason, the use of bad eggs, increases the health risk and reduces the shelf-life of the fresh egg products. The use of incubated or decayed eggs can be detected verifying the presence of some organic acids indicative of metabolic products and microbial decomposition such as succinic acid, lactic acid and D-3-hydroxybutyric acid. The best method currently highlighting this fraud is the test of D-3-hydroxybutyric acid, very low in fresh eggs (<1mg/kg), but much more high in the wastes of incubator. The analysis is generally carried out by extraction and gas chromatography.

D-3-hydroxybutyric + NAD+ frecciaPink complex

The D-3-hydroxybutyric acid reacts, by an enzymatic reaction with the NAD+ and induces the formation of a pink color whose intensity, measured at 505 nm, is directly proportional to the concentration of D-3-hydroxybutyric acid in the sample. The result is expressed as ppm of D-3-hydroxybutyric acid.

Correlation data

Comparative tests on samples of mixed eggs where was add a D-3-hydroxybutyric acid standard, checked with CDR FoodLab® method, confirmed a very good alignment.


Comparative tests on samples of mixed eggs where was add a D-3-hydroxybutyric acid standard, checked with CDR FoodLab® method


Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300420 suitable for 100 tests.

Code *300426 suitable for 10 tests.

The reagents have a shelf-life of 12 months.

Sample Handling

Powdered egg, mixed egg and egg white, yolk diluited. Transfer part of the solution obtained in a centrifuge tube and centrifuge for 5 minutes.

Measuring range

Test Measuring Range Resolution Repeatability
D-3-Hydrox. ac. - egg mixture 0.5 - 15 ppm 0.1 ppm 0.5 ppm
D-3-Hydroxybutyric acid - egg yolk 0.5 - 15 ppm 0.1 ppm 1 ppm

For samples with D-3-hydroxybutyric acid values > 15 ppm, you have to use half the sample volume (75 μL) and multiply the result by 2.

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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