Determination of Lactose in Bakery Products and Baked Goods

CDR FoodLab®: the analysis system to perform a complete quality control on bakery products, snacks and spreads.

CDR FoodLab® allows you to determine lactose on bakery products and baked goods in order to verify the exact residual amount of lactose present in the lactose-free products.

The sample preparation is very easy and it is carried out in 4 easy steps:

  • Grind the sample and weigh it as it is written in the method
  • Add a specific amount of water
  • Homogenise
  • Centrifuge and collect the supernatant to carry out the analysis

In 10 minutes with this easy method you can obtain affordable and precise results as you obtain with the reference method (HPLC) that needs chemical skill and expertise being also very time consuming.

The Analyzers CDR FoodLab® for a complete quality control on bakery products, baked goods, snacks and spreads.

The system is supplied pre-calibrated and ready for use.
Results are expressed in accordance with the reference method.
The analytical methods are simple: the system does not require an equipped laboratory or skilled staff.

  CDR FoodLab® for quality control on bakery products, snacks and spreads.CDR FoodLab® CDR FoodLab® Junior for quality control on bakery products, snacks and spreads.CDR FoodLab® Junior
Analyses Bakery Products and Spreads:
Free Fatty Acids
Peroxide Value
Anisidine Value (AnV)
Lactose
Alcohol by Volume
Alcohol by Volume on Preservative solution
Configuration with tailored panel of analyses
Simultaneous Sample Analyses 16 3
Multiple Analyses on a Sample si

Correlation curve

Here below we have the correlation of the results obtained doing the analyses with CDR FoodLab® and the reference method on low lactose milk. The correlation R2= 0,9882 is great considering the difficulty of the reference method.

The analysis of lactose on lactose-free milk was performed with CDR FoodLab® and with the HPLC method (ISO 22662) . The correlation R2 = 0.9882

In the case of finished products, with a sample preparation, a limpid supernatant containing lactose  is obtained. The analysis is carried out on this supernatant.

Methods

Test type: End Point
Time of test: 10 minutes
It is possible to carry out several test sessions with a maximum of 16 samples.

Test principle

Lactose is a disaccharide sugar that is found in milk and is formed from galactose and glucose. Lactose makes up around 4.5~5% of milk (by weight). The enzyme lactase is essential for digestive hydrolysis of lactose in milk. Deficiency of the enzyme causes lactose intolerance.

Lactose is split in glucose and galactose. Glucose reacts with a phenolic compound through an enzymatic reaction, with peroxidase, and forms a pink coloured complex. The absorbance of the complex is read at 505 nm, and the value is directly proportional to the concentration of lactose in the sample.

Reagent test Kits

The reagents are packaged in foil pouches containing 10 tubes useful to perform 10 analyses.

This is the minimum package that allows the use of CDR systems even to those who need to make a few analyses, thus not wasting reagents.

There are also boxes of 100 tests, however, packaged in 10 bags of 10 tubes containing the reagent.

Code *300015: 100 tests

Code *300010: 10 tests

The reagents have a shelf-life of 12 months

Sample Preparation

A grinded product is weight and it is added a define amount of distilled water. After the centrifugation the supernatant obtained is collected for the analysis

Measuring range

Test Measuring Range Resolution Repeatability
Lactose [0.01-2 g/100g] 0.01 - 2 g/100g 0.01 g/100g 0.05 g/100g

Reagents developed by CDR S.r.l - Quality System Certified ISO 9001 Ed. 2015 - Certificate n. 9115

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With the CDR system, you can perform your tests in total autonomy

Neither skilled staff nor a dedicated external laboratory is needed to use our systems. CDR FoodLab® optimizes traditional testing methods, making them faster and easier, while guaranteeing accuracy standards in line with the reference methods.

Analytical kit

CDR FoodLab® Analysis Systems use disposable pre-vialed reagents specifically developed by the research laboratories of CDR. The use of pre-vialed reagents and the analytical procedures allow:

  • a sample preparation that is quick and easy, when needed at all;
  • making analytical procedures extremely fast and easy;
  • removing all needs for complex calibration procedures.

Reagents come in aluminum packages containing 10 test tubes each to perform 10 tests (or packages for 100 tests containing 10 single packages of 10 test tubes each).

Just a few steps and results are yielded right away

Just a few steps will suffice to carry out your analysis in a rapid way in total autonomy. The system is composed of both the analyzer based on photometric technology and a kit of low toxicity, pre-vialed reagents, developed by CDR.

CDR FoodLab® is cost-effective because it does not require the use of expensive equipment and does not entail maintenance, assistance or calibration costs.
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